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Procedures are provided in the supplementary materials. A total of 157 individuals were genotyped. Data were obtained from fasting blood samples, taken in the morning before breakfast, following an overnight fast and 12 h after, as well as from standard clinical investigations. The study was approved by the local ethics committee and was performed in accordance with the Declaration of Helsinki. All participants provided written informed consent. All participants were Caucasians. The DNA extraction was performed using the QIAamp DNA Blood Mini Kit according to the manufacturer’s instructions (QIAGEN, Chatsworth, CA). DNA concentration was quantified by spectrophotometry (Nanodrop; Thermo Scientific, Waltham, MA). Each DNA sample was diluted to 50 ng/mL and stored at −20°C until the SNP genotyping was performed. All single nucleotide polymorphisms were genotyped by the KBioscience Competitive Allele-Specific PCR Kit (AB) (KBioscience, Hoddeston, Herts, UK) according to the manufacturer’s instructions. The SNP genotyping was performed using the KASPar PCR Master Mix (KBioscience), with the primer sequences and corresponding conditions provided in the supplementary materials. The data were processed using Sequenom MassARRAY® Typer 4.0 software (Sequenom, San Diego, CA). The genotypic and allelic frequencies of each SNP were then analysed. The Hardy-Weinberg equilibrium was calculated using the chi-squared test. Single-marker association analysis and the linkage disequilibrium (LD) structure was performed by Haploview software [31]. Haplotype-based association was performed using the EM algorithm. The polymorphism c.1020*+718G>A was analysed for association with the methadone detoxification outcome using multivariate logistic regression analysis. The test for association was performed using the univariate and multivariate logistic regression analysis with adjustment for variables related to methadone detoxification, such as age, length of treatment, number of relapses or follow-up duration. The odds ratios (OR) and 95% confidence intervals (CI) were used to describe the strength of association. The significance level was set at 0.05. The statistical analysis was performed using SAS software (v.9.1) (SAS Institute Inc., Cary, NC).

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